201*ȺڌվйY(ji)
ڌվ201*ȿйY(ji)
201*ǺڌվĿйȡÿԽʽl(f)չһꡣ@һĿՓĶȡһijɿmȻ@ЩɿǺܴnjںڌվǾͻơ
ڌվԺI(lng)(do)P(gun)c֧£^ȥһĹ(dng)ڌվI(lng)(do)MıMڌվI(y)l(f)չڵĺˮվI(lng)(do)I(lng)ڌվȫwT˷kh(hun)ɵIJڿĿĿ(zh)еȷSȡ^гЧĘI(y)挦P(gun)rMпY(ji)
һ201*Ŀ@͈(zh)r
ڌվ201*@ÿ@Ŀ6
Ѓ헞뼉ԿԺИI(y)(w)Mքe飺1Q㽶a(chn)P(gun)Ig(sh)оĿԴ_ԺĿĿ(jng)M30fԪ2Q߾c㽶ϵķәCĿԴh(hun)cֲﱣoо_ԺĿĿ(jng)M20fԪ
ʡȻƌW(xu)Qքe飺ʡ㽶{(dio)鼰ԭϵb㽶ѪӵķxͽY(ji)(gu)bоGFPʾۙо㽶߾Ⱦ㽶ϵ^
ʡcƼĿ1헣㽶ˮһwЧ༼g(sh)ԇоcʾĿ(zh)rĿӋҪքeژ|~ȵز㽶w(x)sо㽶ˮʸЧüg(sh)о㽶{(dio)鼰l(f)Ҏ(gu)оÃ(ni)(x)㽶ήо㽶o~ЙCʵƼ䑪(yng)о㽶ӹP(gun)Ig(sh)оȵP(gun)ĿǰMСĿҲMЮ(dng)ȡһĽY(ji)
201*Ŀ@r
201*ȺڌվĿ@5ĿЇҡ948Ŀ2Ŀρ1헣ρИI(y)(w)M2н(jng)M_200fԪ@ЩĿī@ڌվ_չĿй춨ˈԌĻA(ch)w飺
һҡ948Ŀ2
1ĿQ㽶wS坍ÓzˇƷP(gun)Ig(sh)MMM㽶wSøÓzg(sh)ϵӡȿƌW(xu)cI(y)оίT\g(sh)ParukuttyammaPremaParukuttyammaPremaҪоI(lng)ﻯW(xu)Ͱl(f)
2ĿQ㽶NP(gun)Ig(sh)MĿ(ni)ݣMg(sh)ɷ棺
1㽶Ng(sh)㽶Ҹ(x)B(yng)g(sh)ֲwȡIJλͬAεB(yng)䷽B(yng)rgwߵT(do)ȣ㽶ԭ|(zh)wB(yng)g(sh)(ϵĻȡIJλøƄxPHֵBo(x)x(x)ںϵ)zD(zhun)g(sh)ͬD(zhun)wr(nng)UȾrg|(zh)dwĘ(gu)ӵx늓ȣ
2ܻ
Replicase-associatedprotein(Rep)gene(f)øρ1
ԓĿxַںʡ㽶a(chn)^(q)Y(ji)ϱλČHr͇㽶a(chn)I(y)g(sh)wϵھCԇվİl(f)չĿ(bio)΄(w)ҪO(sh)㽶(bio)(zhn)ʾ@^(q)ַxСIJĿݘ(bio)(zhn)a(chn)ʾ^(q)150IJ(bio)(zhn)a(chn)ʾ^(q)100ԓʾ^(q)Й(qun)`҆λO(sh)(bio)(zhn)ʾ@250ݘ(bio)(zhn)ʾ@Ҫ㽶CB(yng)og(sh)(ji)ˮ(ji)ʼg(sh)IJ(bio)(zhn)ʾ@^(q)ҪԹ(ji)ˮ(ji)ʼg(sh)˰bˮһwιʩϵy(tng)cǰڱλоIJĿƶ(yn)oor(nng)´ʩA(y)ӋĿʩ5gʾ201*ݗƏVe_2fӖ(xn)I(y)g(sh)a(chn)ƏVˆT500˴ͨ^(bio)(zhn)ʾ@O(sh)r(nng)w㽶(bio)(zhn)a(chn)g(sh)Ӯa(chn)߹Ʒ|(zh)(ji)sɱ߽(jng)ЧČЧ߀r(nng)ˆTr(nng)(bio)(zhn)a(chn)RF(xin)㽶a(chn)g(sh)ˮƽ
ρИI(y)(w)M2
1ĿQ㽶ǴxƷ|(zh)γc{(dio)õо
Ŀ(ni)ݣͨ^˽㽶l(f)^йMǷeۡ|(zh)ɫغ̓(ni)Դص׃Ҏ(gu)̽ӑ㽶l(f)^Ѓ(ni)ɷּҪxøSC|(zh)V|(zh)Ԫصȵ׃Ҏ(gu)ɺP(gun)ϵͨ^ˮƽо{(dio)㽶Ʒ|(zh)ṩՓ(j)2ĿQw{(dio){(dio)(ji)㽶ɺƷ|(zh)о
Ŀ(ni)ݣĿᘌ㽶п¡ĹȰÓøGlutamatedecarboxylase,GAD,MaGAD1͙ϳøGlyoxysomalcitratesynthaseGCSMaGCSо㽶^е{(dio)üCҪо(ni)Ѓɂ棺1ϩ1-MCPȷʽ̎㽶оԓڸ̎ı_ʽ2w{(dio)عȰÓø͙ϳøı_оcƷ|(zh)γɵP(gun)ϵͬϩ㽶r·
201*Ƽlƽ_O(sh)r
ڌվ201*ُĿr
201*(zh)Ѓ헫@ĿЗlƽ_O(sh)Ŀ㽶W(xu)c죬IJ㽶a(chn)I(y)g(sh)c(chung)»ػA(ch)O(sh)ʩ@ɂĿMЮ(dng)A(y)Ӌ201*깤ƺڌվĿkl
ġƼƏVc(w)r(nng)r
J(rn)䌍ЇᎧr(nng)I(y)ƌW(xu)ԺƼMMвпhr(nng)յľͬr`пƌW(xu)l(f)չ^(w)r(nng)Ŀ(bio)ڌվГ(dn)r(nng)I(y)Ƽ˾P(gun)r(nng)ƏVcwϵO(sh)M(yu)r(nng)a(chn)Ʒشg(sh)ƏVʾhr(nng)ˆTӖ(xn)ĿĿʩMᎧI(lng)Č_չr(nng)I(y)Ƽl(xing)Ӗ(xn)߀c(zi)Ȟ(zi)Y(ji)ϣ_L(fng)Σڱȵķ(w)r(nng)Ӗ(xn)r(nng)ˆT201*l(f)ſƼY8000(w)w14пhm(x)ķ(w)r(nng)ԌA(ch)
Ƽ
ںڌվĚvʷԭзĹ]ȱڿй
Ľ(jng)ǺڌվԿƌW(xu)l(f)չ^ָ(do)˼˞鱾đB(ti)ȣJ(rn)W(xu)(x)bȡІλڿйijɹ(jng)c֮̎ڌվİl(f)չD(zhun)͵춨ĹA(ch)
201*ڌվɳP(gun)ˆTW(xu)(x)Ӗ(xn)Ҫ㽶ή²CزԌW(xu)g(sh)ӑ։^(q)y䷽ʩʹӑ(zhn)r(nng)I(y)졢㽶a(chn)I(y)g(sh)wϵg(sh)Ӗ(xn)㽶a(chn)I(y){(dio)йˮһwg(sh)Ӗ(xn)r(nng)Ar(nng)I(y)ƼӖ(xn)ϾӢZӖ(xn)Ϯa(chn)I(y){(dio)е@һꮔ(dng)пƼˆTѰl(f)SCIՓ2ƪĺڿ2ƪ
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2ȱٿĿ
wƵĆ}ȱP(gun)Ŀ֧ĶȱІӽ(jng)MoӋ_չP(gun)Ŀл
3лؽO(sh)
ՈĿkҸ졢ؽO(sh)P(gun)(jng)M߀δλӰ˿лA(ch)lO(sh)M
4
ںڌվl(f)չĚvʷԭ˲鷽Omˆλl(f)չҪMP(gun)˲enjW(xu)Ǝ^˵M
201*깤Ӌ
@O(sh)һоİl(f)չĿ(bio)㽶a(chn)I(y)l(f)չӏվƼA(ch)O(sh)ʩ̓xb佨O(sh)eOcĿȡԿĿӆλl(f)չԆλl(f)չ(do)㽶a(chn)I(y)(gu)ϿƼl(f)չҎ(gu)ɵĿЄ(chung)wϵ͌wϵI(lng)֧κͷ(w)㽶a(chn)I(y)A(ch)о¼g(sh)оͮa(chn)Ʒаl(f)ȫߺڌվ(chung)㽶a(chn)I(y)ṩԭʼ(chung)¼g(sh)ƽ_ṩشg(sh)аl(f)ƽ_ṩƼɹD(zhun)ƽ_ṩ⽻Mل(chung)¼g(sh)ƽ_M҇㽶a(chn)I(y)
(j)wl(f)չĿ(bio)201*깤Ӌ1лA(ch)lO(sh)
cץ㽶W(xu)cҺͣIJ㽶a(chn)I(y)g(sh)c(chung)»ػA(ch)O(sh)ʩĿĽO(sh)ӴxO(sh)估P(gun)O(sh)ʩͶһڌվİl(f)չD(zhun)䁉|
2Ŀ
ڌվŬ˷ڙCԭÿĿ乤eOȡĿĿ֧eOcȻƌW(xu)ρ헡ʡԺI(y)(w)MĿ(j)㽶a(chn)I(y)еĆ}͆λr҂Ŭ¹
1J(rn)҇㽶a(chn)I(y)F(xin)cl(f)չڅݵоᘌ㽶a(chn)I(y)ĿǰڵһЩcP(gun)}TṩO(sh)Ҋca(chn)I(y)P(gun)І}҂һĿṩزġ
2MаŽٷN|(zh)YԴռͱȫ㽶N|(zh)YԴԣN|(zh)YԴMurc(chung)
3ӏcλҵĽceOMcƏV㽶ӹȵMg(sh)Ӌ201*cӡȇ㽶оӡDorven˾mRDW(xu)оϓINIBAPȇM㽶YԴϢϵy(tng)ήa(chn)Ʒ_l(f)á㽶cԭӻо㽶Nֲg(sh)Ӗ(xn)㽶YԴھȷČW(xu)(x)c
4ӏ㽶Ʒ|(zh)о҇㽶a(chn)I(y)l(f)չṩՓ(j)5ӏ㽶оeᘌ㽶ή6Mȫ(ni)㽶ƷNͼg(sh)ƏVcӖ(xn)3ӏ齨O(sh)eOM˲
ĺl(f)չǿІλl(f)չĺǺڌվĿǰڌW(xu)Ǝ^˘OδڌվĿI(y)l(f)չҪߌӴεĿ˲҂Ӌ201*M9TʿW(xu)λϵP(gun)ˆTM
4ӏĿ
ҪǼӏճԃ(w)ӏĿڙzȹмӏˆT֮gW(xu)g(sh)ĿоӋMȵȅRӏW(xu)g(sh)ʹƶȻ
ڌվk
201*.
Uչx201*cҹY(ji)V
3
(Q)c
201*ȹY(ji)
QІλw£
Σˣڣ
201*cҹY(ji)V
һһr
ҵĶλоһ팍ҽO(sh)Mչ
rҶλҪоcĿ(bio)ҵĵλԼڿƼl(f)չõȣаl(f)Ӻͳɹr
1Г(dn)΄(w)śrГ(dn)΄(w)(sh)(jng)M(sh)Ҽ
ʡмĿrĿMչr2оɹśr
3Կгɹ飨^5헣
ԿгɹɰA(ch)о(yng)ûA(ch)оͻA(ch)ԹҪܴ팍ҵĿЌ͌W(xu)g(sh)ˮƽ䌦(jng)Ƽl(f)չҪؕIϵйa(chn)شоɹ
ՈҪBԿгɹҪ(ni)ҪĿƼ(chung)ؕI(ni)Ӱ푵ҪC(j)
Ոÿ헴Գɹĺ鼰P(gun)ɹď(f)ӡɹˮƽӰ푵CϵbӆɃ鸽һύ齨O(sh)c˲B(yng)
1Y(ji)(gu)cFO(sh)ՈҪB齨O(sh)
B(yng)reLjFMB(yng)(ni)⃞(yu)˲ŵĴʩȡõijɿ
2κ͌W(xu)g(sh)^˼(yu)Ǹɺ飺ՈκҪBW(xu)g(sh)^˺̓(yu)Ǹɵr
(yu)Ǹɣ45qָҽMԁ̶
ˆTЫ@ÇȻƌW(xu)ҿƼM͇Ҽg(sh)l(f)ȪϿгɹҪߣǰ5@ÇҽܳƌW(xu)ߣ863973ϯƌW(xu)ңʡƌW(xu)g(sh)صȪһȪϿгɹҪߣǰ5ʡ(chung)FˆT(chung)˲ū@_Žc\й1⽻cr
2ƽ_rՈҪB̓xO(sh)ʹ_
ź̶ȣ_l(f)O(sh)fO(sh)ȷr
3\йrՈҪB҃(ni)Ҏ(gu)ƶȽO(sh)
ճW(xu)g(sh)ίTãҿзՇ͌W(xu)g(sh)L(fng)Пo`W(xu)g(sh)µ¼l(f)
4Іλ֧rcІλP(gun)ϵcԺϵ
P(gun)ϵǷؔĿЌwІλǷo茍ҪĽеČҿgֵºؔ(w)(qun)ش헽(jng)Mֵ֧ȷrĿǰچ}һİl(f)չ˼·ߡcҰl(f)չh
ʾP(gun)ڡ201*ȺڌվйY(ji)oķHչ˼Sʹã201*ȺڌվйY(ji)ԓƪ½h(chung)
ԴW(wng)j(lu) ؟(z)ăHތW(xu)(x)a(chn)(qun)}Ո(lin)ϵ҂rh